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Which means - if you are looking for a solution to a problem and you want to research separation theory - a great book.
If your looking for a quick fix to an HPLC problem - not as good. This is a detailed, extensive and well written text on HPLC Method development - wish there was more on validation here though.
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The approach is the same as that in the first edition: fluorescence spectroscopy from the viewpoint of a biophysical chemist. Explanations are clear. References are profuse; they now number well over a thousand and include an additional appendix that cites many books and review articles for additional reading. Figures are also plentiful; no fancy color diagrams a la contemporary biochemistry texts, but basic line drawings, primarily adapted from journal articles.
The first three chapters ("Introduction to Fluorescence", "Instrumentation for Fluorescence Spectroscopy", and "Fluorophores") are an excellent brief (93-page) introduction to the whole field. Most of the remaining 19 chapters are organized around the kinds of photophysical processes and spectroscopic quantities that are relevant to fluorescence. These include very thorough treatments of fluorescence lifetimes, quenching, anisotropy, and energy transfer. There are many examples drawn from journal articles. In addition, the work now covers more of the technological applications of fluorescence, especially in chapters on "Fluorescence Sensing" and "DNA Technology". These give a sense of the great contributions that fluorescence has made to biotechnology.
My own work involves varied applications of fluorescence to biotechnology, and the second edition of Lakowicz is my reference book of choice. It would also be an excellent text for a graduate course on fluorescence in a chemistry or biochemistry department. All in all, this is a most worth successor to the first edition.
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Roth is able to tell us so much history in so brief a space. Here are the beginnings of Isherwood's BERLIN STORIES, the birth of the style of the recent works of WG Sebald's books, and even the writings of Edmund White in THE FLANEUR. Would that our newspapers could find the space AND the talent to place such insightful observations in our poetically vapid journalism of today! This is a rare book of beautiful writing and we are indebted to translator Michael Hofmann not only for his lyrical English style, but also for his own insightful essay about the man who wrote these 'feuilletons'. A sad parting note is that Joseph Roth died in Paris in 1939 from the effects of his alcoholism. Such was the influence of Berlin on many artists of thetime.
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